Complete biosynthetic pathways of ascofuranone and ascochlorin in Acremonium egyptiacum.

ABSTRACT: Ascofuranone (AF) and ascochlorin (AC) are meroterpenoids produced by various filamentous fungi, including Acremonium egyptiacum (synonym: Acremonium sclerotigenum), and exhibit diverse physiological activities. In particular, AF is a promising drug candidate against African trypanosomiasis and a potential anticancer lead compound. These compounds are supposedly biosynthesized through farnesylation of orsellinic acid, but the details have not been established. In this study, we present all of the reactions and responsible genes for AF and AC biosyntheses in A. egyptiacum, identified by heterologous expression, in vitro reconstruction, and gene deletion experiments with the aid of a genome-wide differential expression analysis. Both pathways share the common precursor,

SUBMITTER: Araki Y 

PROVIDER: S-EPMC6486709 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature