Extreme mechanical diversity of human telomeric DNA revealed by fluorescence-force spectroscopy.

ABSTRACT: G-quadruplexes (GQs) can adopt diverse structures and are functionally implicated in transcription, replication, translation, and maintenance of telomere. Their conformational diversity under physiological levels of mechanical stress, however, is poorly understood. We used single-molecule fluorescence-force spectroscopy that combines fluorescence resonance energy transfer with optical tweezers to measure human telomeric sequences under tension. Abrupt GQ unfolding with K⁺ in solution occurred at as many as four discrete levels of force. Added to an ultrastable state and a gradually unfolding state, there were six mechanically distinct structures. Extreme mechanical diversity was also observed with Na⁺, although GQs were mechanically weaker. Our ability to detect small

SUBMITTER: Mitra J 

PROVIDER: S-EPMC6486787 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature