Label-free high-speed wide-field imaging of single microtubules using interference reflection microscopy.

ABSTRACT: When studying microtubules in vitro, label free imaging of single microtubules is necessary when the quantity of purified tubulin is too low for efficient fluorescent labelling or there is concern that labelling will disrupt function. Commonly used techniques for observing unlabelled microtubules, such as video enhanced differential interference contrast, dark-field and more recently laser-based interferometric scattering microscopy, suffer from a number of drawbacks. The contrast of differential interference contrast images depends on the orientation of the microtubules, dark-field is highly sensitive to impurities and optical misalignments. In addition, all of these techniques require costly optical components such as Nomarski prisms, dark-field condensers, lasers and laser scanners. Her

SUBMITTER: Mahamdeh M 

PROVIDER: S-EPMC6486835 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature