Identification of dentinogenic cell-specific surface antigens in odontoblast-like cells derived from adult dental pulp.

ABSTRACT:

Background

Odontoblast is a unique progenitor that plays a role in dentin formation. So far, the dentinogenic differentiation of dental pulp stem cells and the role of surface molecules of odontoblasts in dentinogenesis are not well known yet. In this study, we obtained odontoblast-like cells from human dental pulp cells and screened odontoblast-specific cell surface antigens by decoy immunization.

Methods

Through decoy immunization with intact odontoblast-like cells derived from human dental pulp cells, we constructed 12 monoclonal antibodies (mAbs) of IgG type, and their binding affinities for cell surface of odontoblast-like cells were analyzed by flow cytometry. Immunoprecipitation, mass spectrometry, and immunohistochemistry were performed to demonstrate odontoblast-spe

SUBMITTER: Kang KJ 

PROVIDER: S-EPMC6487011 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature