Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction.

ABSTRACT:

Background

Bone-derived mesenchymal stem cell (BMSC) transplantation has been reported to be effective for the treatment of ischemic heart disease, but whether BMSCs are the optimal cell type remains under debate. Increasing numbers of studies have shown that Nestin, an intermediate filament protein, is a potential marker for MSCs, which raises the question of whether Nestin⁺ cells in BMSCs may play a more crucial role in myocardial repair.

Methods

Nestin⁺ cells were isolated using flow cytometry by gating for CD45^- Ter119^- CD31^- cells from the compact bone of Nestin-GFP transgenic mice, expressing GFP driven by the Nestin promoter. Colony-forming and proliferative curve assays were conducted to determine the proliferati

SUBMITTER: Lu D 

PROVIDER: S-EPMC6487029 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature