High-density linkage map construction and mapping QTL for yield and yield components in autotetraploid alfalfa using RAD-seq.

ABSTRACT:

Background

Alfalfa (Medicago sativa L.) is an important forage crop grown worldwide. Alfalfa is called the "queen of forage crops" due to its high forage yield and nutritional characteristics. The aim of this study was to undertake quantitative trait loci (QTL) mapping of yield and yield-related traits in an F₁ population of two alfalfa varieties that differ in their yield and yield-related traits.

Results

We constructed a high-density linkage map using single nucleotide polymorphism (SNP) markers generated by restriction-site associated DNA sequencing (RAD-seq). The linkage map contains 4346 SNP and 119 simple sequence repeat (SSR) markers, with 32 linkage groups for each parent. The average marker distances were 3.00 and 1.32 cM, with coverages of 3455 cM and 43

SUBMITTER: Zhang F 

PROVIDER: S-EPMC6487053 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature