Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016.

ABSTRACT:

Aim

This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains.

Materials and methods

We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences.

Results

Our molecular surveillance revealed that 31.6% of the tested specimens we

SUBMITTER: Alsultan MA 

PROVIDER: S-EPMC6487242 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature