Multiplex clonal analysis in the chick embryo using retrovirally-mediated combinatorial labeling.

ABSTRACT: Lineage analysis plays a central role in exploring the developmental potential of stem and progenitor cell populations. In higher vertebrates, a variety of techniques have been used to label individual cells or cell populations, including interspecies grafting, intracellular microinjection, and Cre-mediated recombination. However, these approaches often suffer from difficulties in progenitor cell targeting, low cellular resolution and/or ectopic labeling. To circumvent these issues, here we utilize replication incompetent avian (RIA) retroviruses to deliver combinations of fluorescent proteins into distinct cellular compartments in chick embryos. In particular, RIA-mediated lineage tracing is optimal for long term mapping of dispersing cell populations like the neural crest. Using this too

SUBMITTER: Tang W 

PROVIDER: S-EPMC6487888 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature