PulmoBind Imaging Measures Reduction of Vascular Adrenomedullin Receptor Activity with Lack of effect of Sildenafil in Pulmonary Hypertension.

ABSTRACT: Endothelial dysfunction is a core pathophysiologic process in pulmonary arterial hypertension (PAH). We developed PulmoBind (PB), a novel imaging biomarker of the pulmonary vascular endothelium. ^99mTechnetium (^99mTc)-labelled PB binds to adrenomedullin receptors (AM₁) densely expressed in the endothelium of alveolar capillaries. We evaluated the effect of sildenafil on AM₁ receptors activity using ^99mTc-PB. PAH was induced in rats using the Sugen/hypoxia model and after 3 weeks, animals were allocated to sildenafil (25 or 100 mg/kg/day) for 4 weeks. ^99mTc-PB uptake kinetics was assessed by single-photon emission computed tomography. PAH caused right ventricular (RV) hypertrophy that was decreased by low and high sildenafil d

SUBMITTER: Merabet N 

PROVIDER: S-EPMC6488585 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature