UM171 expands distinct types of myeloid and NK progenitors from human pluripotent stem cells.

ABSTRACT: Scaling up blood cell production from hPSCs is critical to advancing hPSC technologies for blood transfusion, immunotherapy, and transplantation. Here we explored the potential of the HSC agonist pyrimido-indole derivative UM171, to expand hematopoietic progenitors (HPs) derived from hPSCs in chemically defined conditions. We revealed that culture of hPSC-HPs in HSC expansion conditions (SFEM with added TPO, SCF, FLT3L, IL3 and IL6) in the presence of UM171 predominantly expanded HPs with a unique CD34⁺CD41a^loCD45⁺ phenotype that were enriched in granulocytic progenitors (G-CFCs). In contrast, in lymphoid cultures on OP9-DLL4, in the presence of SCF, FLT3L, and IL7, UM171 selectively expanded CD34⁺CD45⁺CD7⁺ lymphoid progenit

SUBMITTER: Mesquitta WT 

PROVIDER: S-EPMC6488662 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature