CUT&Tag for efficient epigenomic profiling of small samples and single cells.

ABSTRACT: Many chromatin features play critical roles in regulating gene expression. A complete understanding of gene regulation will require the mapping of specific chromatin features in small samples of cells at high resolution. Here we describe Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components. In CUT&Tag, a chromatin protein is bound in situ by a specific antibody, which then tethers a protein A-Tn5 transposase fusion protein. Activation of the transposase efficiently generates fragment libraries with high resolution and exceptionally low background. All steps from live cells to sequencing-ready libraries can be performed in a single tube on the benchtop or a micr

SUBMITTER: Kaya-Okur HS 

PROVIDER: S-EPMC6488672 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature