VirGenA: a reference-based assembler for variable viral genomes.

ABSTRACT: Characterization of the within-host genetic diversity of viral pathogens is required for selection of effective treatment of some important viral infections, e.g. HIV, HBV and HCV. Despite the technical ability of detection, there are conflicting data regarding the clinical significance of low-frequency variants, partially because of the difficulty of their distinguishing from experimental artifacts. The issue of cross-contamination is relevant for all highly sensitive techniques, including deep sequencing: even trace contamination leads to a significant increase of false positives in identified SNVs. Determination of infections by multiple genotypes of some viruses, the incidence of which can be considerable, especially in risk groups, is also clinically significant in some cases. We deve

SUBMITTER: Fedonin GG 

PROVIDER: S-EPMC6488938 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature