Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool.

ABSTRACT:

Background

Artificial induction of mutagenesis is effective for genetic resource innovation and breeding. However, the traditional mutation methods for fish breeding are not convenient or safe for daily use. Hence, development of a simple, safe and effective mutagenesis method with a high mutation rate and applicability to multiple fish species, is needed.

Results

We reported the first successful mutagenesis in a marine aquaculture fish species, Japanese flounder, Paralichthys olivaceus, using a novel atmosphere and room temperature plasma (ARTP) mutagenesis tool. ARTP treatment time was optimized for the fertilized eggs and sperm, respectively. Eggs fertilized for 60 min were treated by ARTP with a radio-frequency power input of 120 W, and the ARTP treatment time was 25 min

SUBMITTER: Ji-Lun H 

PROVIDER: S-EPMC6489211 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature