Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells.

ABSTRACT:

Background

During decidualization in endometrial stromal cells (ESCs), expressions of a number of genes and epigenetic modifications of histones are altered. However, there is little information about whether DNA methylation, which is another epigenetic mechanism, also changes during decidualization. Here, we examined the genome-wide DNA methylation profiles in ESCs during decidualization and their associations with the changes of gene expressions and histone modifications.

Results

ESCs were incubated with estradiol and medroxyprogesterone acetate for 14 days to induce decidualization. The genome-wide DNA methylation profiles were compared between the non-decidualized ESCs and the decidualized ESCs. Of 482,005 CpGs, only 23 CpGs (0.0048%) showed different DNA methylation sta

SUBMITTER: Maekawa R 

PROVIDER: S-EPMC6489213 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature