Eliminating contraction during culture maintains global and local Ca²⁺ dynamics in cultured rabbit pacemaker cells.

ABSTRACT: Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca²⁺ signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K⁺ channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in pacemaker cells can only be assessed if the Ca²⁺ dynamics are in the physiological range. Cultured cells that can be genetically manipulated and/or virally infected with probes are required for this purpose. Because rabbit pacemaker cells in culture experience a decrease in their spontaneous action potential (AP) firing rate below the physiological range, Ca²⁺ dynamics are expected to be affected. However, Ca²⁺ dynamics in

SUBMITTER: Segal S 

PROVIDER: S-EPMC6489480 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature