Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor.

ABSTRACT: The SELEX (Systematic Evolution of Ligands by EXponential enrichment) method has been used successfully since 1990, but work is still required to obtain highly specific aptamers. Here, we present a novel approach called 'Competitive non-SELEX' (and termed as 'SELCOS' (Systematic Evolution of Ligands by COmpetitive Selection)) for readily obtaining aptamers that can discriminate between highly similar targets. This approach is based on the theoretical background presented here, in which under the co-presence of two similar targets, a specific binding type can be enriched more than a nonspecifically binding one during repetitive steps of partitioning with no PCR amplification between them. This principle was experimentally confirmed by the selection experiment for influenza virus subtype-spe

SUBMITTER: Kushwaha A 

PROVIDER: S-EPMC6491428 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature