Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells.

ABSTRACT: Microinjection is an effective actuation technique used for precise delivery of molecules and cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into single cells. Several microinjection techniques have been developed for actuating droplets and cells. However, they are still time-consuming, have shown limited success, and are not compatible with the needs of high-throughput (HT) serial microinjection. We present a new passive microinjection technique relying on pressure-driven fluid flow and pulsative flow patterns within an HT droplet microfluidic system to produce serial droplets and manage rapid and highly controlled microinjection into droplets. A microneedle is secured within the injection station to confine droplets during the microinjection. The co

SUBMITTER: Azarmanesh M 

PROVIDER: S-EPMC6491429 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature