Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.

ABSTRACT: Bacteria show sophisticated control of their cellular organization, and many bacteria deploy different polar landmark proteins to organize the cell pole. Super-resolution microscopy, such as Photo-Activated Localization Microscopy (PALM), provides the nanoscale localization of molecules and is crucial for better understanding of organization and dynamics in single-molecule. However, analytical tools are not fully available yet, in particular for bacterial cell biology. For example, quantitative and statistical analyses of subcellular localization with multiple cells from multiple fields of view are lacking. Furthermore, brightfield images are not sufficient to get accurate contours of small and low contrast bacterial cells, compared to subpixel presentation of target molecules. Here we des

SUBMITTER: Altinoglu I 

PROVIDER: S-EPMC6491441 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature