Comparative analysis of serologic cross-reactivity using convalescent sera from filovirus-experimentally infected fruit bats.

ABSTRACT: With the exception of Reston and Bombali viruses, the marburgviruses and ebolaviruses (family Filoviridae) cause outbreaks of viral hemorrhagic fever in sub-Saharan Africa. The Egyptian rousette bat (ERB) is a natural reservoir host for the marburgviruses and evidence suggests that bats are also natural reservoirs for the ebolaviruses. Although the search for the natural reservoirs of the ebolaviruses has largely involved serosurveillance of the bat population, there are no validated serological assays to screen bat sera for ebolavirus-specific IgG antibodies. Here, we generate filovirus-specific antisera by prime-boost immunization of groups of captive ERBs with all seven known culturable filoviruses. After validating a system of filovirus-specific indirect ELISAs utilizing infectious-bas

SUBMITTER: Schuh AJ 

PROVIDER: S-EPMC6491471 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature