Evaluating and Correcting Inherent Bias of microRNA Expression in Illumina Sequencing Analysis.

ABSTRACT: microRNA (miRNA) expression profiles based on the highly powerful Illumina sequencing technology rely on the construction of cDNA libraries in which adaptor ligation is known to deeply favor some miRNAs over others. This introduces erroneous measurements of the miRNA abundances and relative miRNA quantities in biological samples. Here, by using the commercial miRXplore Universal Reference that contains an equimolar mixture of 963 animal miRNAs and TruSeq or bulged adaptors, we describe a method for correcting ligation biases in expression profiles obtained with standard protocols of cDNA library construction and provide data for quantifying the true miRNA abundances in biological samples. Ligation biases were evaluated at three ratios of miRNA to 3'-adaptor and four numbers of polymerase c

SUBMITTER: Baroin-Tourancheau A 

PROVIDER: S-EPMC6491513 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature