Targeting mechanotransduction mechanisms and tissue weakening signals in the human amniotic membrane.

ABSTRACT: Mechanical and inflammatory signals in the fetal membrane play an important role in extracellular matrix (ECM) remodelling in order to dictate the timing of birth. We developed a mechanical model that mimics repetitive stretching of the amniotic membrane (AM) isolated from regions over the placenta (PAM) or cervix (CAM) and examined the effect of cyclic tensile strain (CTS) on mediators involved in mechanotransduction (Cx43, AKT), tissue remodelling (GAGs, elastin, collagen) and inflammation (PGE₂, MMPs). In CAM and PAM specimens, the application of CTS increased GAG synthesis, PGE₂ release and MMP activity, with concomitant reduction in collagen and elastin content. Co-stimulation with CTS and pharmacological agents that inhibit either Cx43 or AKT, differentially inf

SUBMITTER: Barrett DW 

PROVIDER: S-EPMC6491562 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature