The transgenerational effects of oocyte mitochondrial supplementation.

ABSTRACT: Many women suffer from either failed fertilisation or their embryos arrest early during development. Autologous mitochondrial supplementation has been proposed as an assisted reproductive technology to overcome these problems. However, its safety remains to be tested in an animal model to determine if there are transgenerational effects. We have supplemented oocytes with autologous populations of mitochondria to generate founders. We mated the female founders and their offspring to produce three generations. We assessed litter size, the ovarian reserve, and weight gain and conducted a full histopathological analysis from each of the three generations. Across the generations, we observed significant increases in litter size and in the number of primordial follicles in the ovary matched by c

SUBMITTER: St John JC 

PROVIDER: S-EPMC6491721 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature