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Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.

ABSTRACT: A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name "Therminator DNA polymerase" has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.

SUBMITTER: Gardner AF 

PROVIDER: S-EPMC6491775 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.

Gardner Andrew F AF   Jackson Kiserian M KM   Boyle Madeleine M MM   Buss Jackson A JA   Potapov Vladimir V   Gehring Alexandra M AM   Zatopek Kelly M KM   Corrêa Ivan R IR   Ong Jennifer L JL   Jack William E WE  

Frontiers in molecular biosciences 20190424

A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name "Therminator DNA polymerase" has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation. ...[more]

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