Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment.

ABSTRACT:

Background

Glioblastoma (GBM), a highly malignant brain tumor, invariably recurs after therapy. Quiescent GBM cells represent a potential source of tumor recurrence, but little is known about their molecular underpinnings.

Methods

Patient-derived GBM cells were engineered by CRISPR/Cas9-assisted knock-in of an inducible histone2B-GFP (iH2B-GFP) reporter to track cell division history. We utilized an in vitro 3D GBM organoid approach to isolate live quiescent GBM (qGBM) cells and their proliferative counterparts (pGBM) to compare stem cell properties and therapy resistance. Gene expression programs of qGBM and pGBM cells were analyzed by RNA-Seq and NanoString platforms.

Findings

H2B-GFP-retaining qGBM cells exhibited comparable self-renewal capacity but higher therapy

SUBMITTER: Tejero R 

PROVIDER: S-EPMC6491796 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature