Tracing of Afferent Connections in the Zebrafish Cerebellum Using Recombinant Rabies Virus.

ABSTRACT: The cerebellum is involved in some forms of motor coordination and learning, and in cognitive and emotional functions. To elucidate the functions of the cerebellum, it is important to unravel the detailed connections of the cerebellar neurons. Although the cerebellar neural circuit structure is generally conserved among vertebrates, it is not clear whether the cerebellum receives and processes the same or similar information in different vertebrate species. Here, we performed monosynaptic retrograde tracing with recombinant rabies viruses (RV) to identify the afferent connections of the zebrafish cerebellar neurons. We used a G-deleted RV that expressed GFP. The virus was also pseudotyped with EnvA, an envelope protein of avian sarcoma and leucosis virus (ALSV-A). For the specific infectio

SUBMITTER: Dohaku R 

PROVIDER: S-EPMC6491863 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature