Priming of HIV-1-specific CD8⁺ T cells with strong functional properties from naive T cells.

ABSTRACT:

Background

HIV-1-specific CD8⁺ T cells are required for immune suppression of HIV-1 replication and elimination of the associated viral reservoirs. However, effective induction of functional HIV-1-specific CD8⁺ T cells from naïve cells remains problematic in the setting of human vaccine trials. In this study, we investigated priming of functional HIV-1-specific CD8⁺ T cells from naïve cells.

Methods

HIV-1-specific CD8⁺ T cells were primed from naïve T cells of HIV-1-seronegative individuals using TLR4 ligand LPS or STING ligand 3'3'-cGAMP in vitro. We established HIV-1-specific CD8⁺ T cell lines from primed T cells and then investigated functional properties of these cells.

Findings

HIV-1-specific CD8⁺

SUBMITTER: Kuse N 

PROVIDER: S-EPMC6491959 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature