Gingival fibroblasts prevent BMP-mediated osteoblastic differentiation.

ABSTRACT:

Objectives

The inhibitory action of the superficial gingival connective tissues may limit the regenerative potential of alveolar bone in periodontal therapy or dental implant applications. The aims of this study were to investigate the hypothesis that gingival fibroblasts (GF) can inhibit bone morphogenetic protein (BMP)-induced osteoblastic differentiation, to determine their expression of BMP inhibitors, and finally to determine whether reduction of these inhibitors can relieve suppression of osteoblastic differentiation.

Methods

Gingival fibroblasts were co-cultured either directly or indirectly with calvarial osteoblasts to assess alkaline phosphatase inhibitory activity, a marker of osteoblastic differentiation. To test total BMP-inhibitory activity of rat GF, condition

SUBMITTER: Ghuman MS 

PROVIDER: S-EPMC6492095 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature