Local injection of Lenti-Olig2 at lesion site promotes functional recovery of spinal cord injury in rats.

ABSTRACT:

Aims

Olig2 is one of the most critical factors during CNS development, which belongs to b-HLH transcription factor family. Previous reports have shown that Olig2 regulates the remyelination processes in CNS demyelination diseases models. However, the role of Olig2 in contusion spinal cord injury (SCI) and the possible therapeutic effects remain obscure. This study aims to investigate the effects of overexpression Olig2 by lentivirus on adult spinal cord injury rats.

Methods

Lenti-Olig2 expression and control Lenti-eGFP vectors were prepared, and virus in a total of 5 μL (10⁸ TU/mL) was locally injected into the injured spinal cord 1.5 mm rostral and caudal near the epicenter. Immunostaining, Western blot, electron microscopy, and CatWalk analyzes were employed to

SUBMITTER: Tan BT 

PROVIDER: S-EPMC6492694 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature