Downregulation of DEC1 contributes to the neurotoxicity induced by MPP⁺ by suppressing PI3K/Akt/GSK3β pathway.

ABSTRACT:

Aim

Differentiated embryonic chondrocyte gene 1 (DEC1) is involved in the neuronal differentiation and development. The aim of this study is to investigate the role of DEC1 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPP⁺ )-induced PD model.

Methods

The location of DEC1 and tyrosine hydroxylase (TH)-positive neurons were detected by immunofluorescence. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse subacute model of PD was established to evaluate the change of DEC1 expression in midbrain. Then, SH-SY5Y cells were used to investigate the role of DEC1 in MPP⁺ -induced neurotoxicity.

Results

We showed that the co-expressed DEC1 and TH neurons took up more than 80% of the expressed TH neurons in the midbrain of mice. DEC1/TH d

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC6492752 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature