CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker in Diffuse Gliomas.

ABSTRACT:

Aims

To explore the expression, methylation pattern, the prognostic value, and the biological consequences of CAMKK2 in gliomas.

Methods

The expression and methylation pattern of CAMKK2 was inferred and validated from mRNA expression profile (N = 866) and methylation profile (N = 426) of glioma tissue samples, and independent samples were used for further validation by IHC and pyrosequencing. To explore the function of CAMKK2 in gliomas, in vitro studies, colony formation assays and migration and invasion assays were performed.

Results

We found the upregulation of CAMKK2 in high-grade glioma samples was associated with promoter hypomethylation. An elevated expression of CAMKK2 was associated with worse prognosis. By in vitro assays, we demonstrated that CAMKK2 could p

SUBMITTER: Liu DM 

PROVIDER: S-EPMC6492904 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature