Neuronal Soluble Fas Ligand Drives M1-Microglia Polarization after Cerebral Ischemia.

ABSTRACT:

Aims

This study explored sFasL expression in neurons and the potential role of neuronal sFasL in modulating the microglial phenotypes.

Methods

In vivo, middle cerebral artery occlusion (MCAO) was induced in both FasL-mutant (gld) and wild-type (wt) mice. In vitro, primary cortical neuron or microglia or coculture from wt/gld mice was subjected to oxygen glucose deprivation (OGD). sFasL level in the supernatant was evaluated by ELISA. Neuronal-conditioned medium (NCM) or exogenous sFasL was applied to primary microglia with or without FasL neutralizing antibody. Protein expression of JAK2/STAT3 and NF-κB pathways were determined by Western blot. The effect of microglia phenotype from wt/gld mice on the fate of ischemic neurons was further elucidated.

Results

In vivo, c

SUBMITTER: Meng HL 

PROVIDER: S-EPMC6492913 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature