Ginkgolide B Protects Against Ischemic Stroke Via Modulating Microglia Polarization in Mice.

ABSTRACT:

Aim

Ginkgolide B (GB) has shown neuroprotective effect in treating ischemic stroke, related to its property of anti-inflammation. Nevertheless, it is unclear whether GB is able to modulate microglia/macrophage polarization, which has recently been proven to be vital in the pathology of ischemic stroke.

Methods

We performed transient middle cerebral artery occlusion (tMCAO) on C57BL/6J male mice and induced cultured BV2 microglia and primary bone marrow-derived macrophages to be M1/2 phenotype by LPS+ interferon-γ and IL-4, respectively. Immunofluorescence and flow cytometry were used for detecting the specialized protein expression of M1/2, such as CD206 and CD16/32. qPCR was utilized to detect the signature gene change of M1/2.

Results

GB significantly reduced cerebr

SUBMITTER: Shu ZM 

PROVIDER: S-EPMC6492915 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature