Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins.

ABSTRACT: Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding, and immune response stimulated upon infection. Alarm cues are often released in the form of proteins, antimicrobial peptides, and immunoglobulins that provide important insights into bodily function and infection status. Here we outline a noninvasive method to identify potential chemical cues associated with infection in fish by extracting, purifying, and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirm

SUBMITTER: Pawluk RJ 

PROVIDER: S-EPMC6492949 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature