Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches.

ABSTRACT: Since their discovery, riboswitches have been attractive tools for the user-controlled regulation of gene expression in bacterial systems. Riboswitches facilitate small molecule mediated fine-tuning of protein expression, making these tools of great use to the synthetic biology community. However, the use of riboswitches is often restricted due to context dependent performance and limited dynamic range. Here, we report the drastic improvement of a previously developed orthogonal riboswitch achieved through in vivo functional selection and optimization of flanking coding and noncoding sequences. The behavior of the derived riboswitches was mapped under a wide array of growth and induction conditions, using a structured Design of Experiments approach. This approach successfully improved the

SUBMITTER: Kent R 

PROVIDER: S-EPMC6492952 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature