Localization-associated immune phenotypes of clonally expanded tumor-infiltrating T cells and distribution of their target antigens in rectal cancer.

ABSTRACT: The degree and type of T cell infiltration influence rectal cancer prognosis regardless of classical tumor staging. We asked whether clonal expansion and tumor infiltration are restricted to selected-phenotype T cells; which clones are accessible in peripheral blood; and what the spatial distribution of their target antigens is. From five rectal cancer patients, we isolated paired tumor-infiltrating T cells (TILs) and T cells from unaffected rectum mucosa (T_UM) using 13-parameter FACS single cell index sorting. TCRαβ sequences, cytokine, and transcription factor expression were determined with single cell sequencing. TILs and T_UM occupied distinct phenotype compartments and clonal expansion predominantly occurred within CD8⁺ T cells. Expanded TIL clones ide

SUBMITTER: Penter L 

PROVIDER: S-EPMC6492980 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature