Bakkenolide-IIIa Protects Against Cerebral Damage Via Inhibiting NF-κB Activation.

ABSTRACT:

Aims

This study was designed to examine the neuroprotective effects of bakkenolide-IIIa, a major novel compound extracted from the rhizome of P. trichinous.

Methods

Transient focal cerebral damage model in rats and oxygen-glucose deprivation (OGD) in cultured hippocampal neurons were performed. The amount of apoptotic neurons was determined using TUNEL assay. The expressions of Bcl-2, Bax, Akt, ERK1/2, IKKβ, IκBα were measured using Western blot. The nuclear translocation and activation of NF-κB was measured using a fluorescence microscope and electrophoretic mobility shift assay (EMSA).

Results

Bakkenolide-IIIa (4, 8, 16 mg/kg; i.g.) was administered immediately after reperfusion could reduce the brain infarct volume, and the neurological deficit, as well as a high d

SUBMITTER: Jiang Q 

PROVIDER: S-EPMC6493129 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature