Importance of riboflavin kinase in the pathogenesis of stroke.

ABSTRACT:

Aims

To explore risk factors for stroke independent of hypertension and the relationship between riboflavin kinase (RFK) and stroke.

Methods

Gene expression profiling in the brains of spontaneously hypertensive rats (SHR) and stroke-prone spontaneously hypertensive rats (SHRSP) was comparatively analyzed by gene chips. The differentially expressed gene RFK was further verified by q-PCR and Western blot. The protective role of RFK-regulated flavins (including riboflavin, flavin mononucleotide, and flavin adenine dinucleotide) in stroke was observed in middle cerebral artery occlusion (MCAO) mice. Influence of flavins on apoptosis and death in oxygen and glucose deprivation (OGD)-treated neurons was examined by flow cytometry. Bax and Bcl-2 proteins were detected by Western bl

SUBMITTER: Zou YX 

PROVIDER: S-EPMC6493343 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature