Heme oxygenase-1 mediated memorial and revivable protective effect of ischemic preconditioning on brain injury.

ABSTRACT:

Aims

Ischemic preconditioning (IPC) has short-term benefits for stroke patients. However, if IPC protective effect is memorial and the role of the intracellular protective protein heme oxygenase-1 (HO-1) is not known.

Methods

Ischemic preconditioning and the corresponding sham control were achieved by blocking the blood flow of the left internal carotid artery for 20 min and 2 second, respectively, in rats. Both IPC and sham-operated animals were divided into three groups and treated with PBS, the HO-1 inducer hemin, and the HO-1 inhibitor Znpp. Three weeks after IPC, brain ischemia-reperfusion injury was achieved by left middle cerebral artery obstruction for 45 min followed by 24-h reperfusion.

Results

2,3,5-triphenyltetrazolium chloride staining and neurological dy

SUBMITTER: Le LL 

PROVIDER: S-EPMC6493422 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature