MiR-139 inhibits Mcl-1 expression and potentiates TMZ-induced apoptosis in glioma.

ABSTRACT:

Aims

Mcl-1, an antiapoptotic member of the Bcl-2 family, is overexpressed in human glioblastoma, conferring a survival advantage to tumor cells. The mechanisms underlying its dysregulation have not been clarified. In this study, we explored the involvement of micro-RNAs that acted as endogenous sequence-specific suppressors of gene expression.

Methods and results

Using computational and TCGA analysis, we identified miR-139 as being downregulated in glioblastoma in comparison with human brain tissue, as well as possessing a putative target site in Mcl-1 mRNA. Overexpression of miR-139 led to a clear decrease in Mcl-1 expression in gliomas. Reporter assays revealed direct post-transcriptional regulation involving miR-139 and the 3'-untranslated region of Mcl-1. Human glioma ti

SUBMITTER: Li RY 

PROVIDER: S-EPMC6493424 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature