Tenuigenin attenuates α-synuclein-induced cytotoxicity by down-regulating polo-like kinase 3.

ABSTRACT:

Background and aims

Tenuigenin (Ten) is a Chinese herbal extract with antioxidative and antiinflammatory effects on toxin-induced cell models of Parkinson's disease (PD); however, its effects on α-synuclein toxicity-based PD models remain unknown. α-synuclein hyperphosphorylation is a key event in PD pathogenesis and potential target of therapeutic interventions. We tested whether Ten alleviates α-synuclein-induced cytotoxicity via reducing kinases that phosphorylate α-synuclein.

Methods

SH-SY5Y cells transiently transfected with wild-type or A53T mutant α-synuclein were used to evaluate the effect of Ten on the levels of α-synuclein phosphorylation-related kinases. Cells treated with 10 μM Ten for 24 h were measured for viability (proliferation and apoptosis assays) and cel

SUBMITTER: Zhou JX 

PROVIDER: S-EPMC6493467 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature