Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species.

ABSTRACT:

Background

Resident macrophages regulate homeostatic and disease processes in multiple tissues, including the kidney. Despite having well defined markers to identify these cells in mice, technical limitations have prevented identification of a similar cell type across species. The inability to identify resident macrophage populations across species hinders the translation of data obtained from animal model to human patients.

Methods

As an entry point to determine novel markers that could identify resident macrophages across species, we performed single-cell RNA sequencing (scRNAseq) analysis of all T and B cell-negative CD45⁺ innate immune cells in mouse, rat, pig, and human kidney tissue.

Results

We identified genes with enriched expression in mouse renal

SUBMITTER: Zimmerman KA 

PROVIDER: S-EPMC6493978 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature