Epitope Selection for HLA-DQ2 Presentation: Implications for Celiac Disease and Viral Defense.

ABSTRACT: We have reported that the major histocompatibility molecule HLA-DQ2 (DQA1*05:01/DQB1*02:01) (DQ2) is relatively resistant to HLA-DM (DM), a peptide exchange catalyst for MHC class II. In this study, we analyzed the role of DQ2/DM interaction in the generation of DQ2-restricted gliadin epitopes, relevant to celiac disease, or DQ2-restricted viral epitopes, relevant to host defense. We used paired human APC, differing in DM expression (DM^null versus DM^high) or differing by expression of wild-type DQ2, versus a DM-susceptible, DQ2 point mutant DQ2α+53G. The APC pairs were compared for their ability to stimulate human CD4⁺ T cell clones. Despite higher DQ2 levels, DM^high APC attenuated T cell responses compared with DM^null APC after intrac

SUBMITTER: Hung SC 

PROVIDER: S-EPMC6494460 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature