Newfound sex differences in axonal structure underlie differential outcomes from in vitro traumatic axonal injury.

ABSTRACT: Since traumatic axonal injury (TAI) is implicated as a prominent pathology of concussion, we examined potential sex differences in axon structure and responses to TAI. Rat and human neurons were used to develop micropatterned axon tracts in vitro that were genetically either male or female. Ultrastructural analysis revealed for the first time that female axons were consistently smaller with fewer microtubules than male axons. Computational modeling of TAI showed that these structural differences place microtubules in female axons at greater risk of failure during trauma under the same applied loads than in male axons. Likewise, in an in vitro model of TAI, dynamic stretch-injury to axon tracts induced greater pathophysiology of female axons than male axons, including more extensive undulat

SUBMITTER: Dolle JP 

PROVIDER: S-EPMC6495524 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature