CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry.

ABSTRACT:

Background

Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recently Fragaria. In order to inform application of the technology in Fragaria, we targeted the visible endogenous marker gene PDS (phytoene desaturase) in diploid Fragaria vesca ssp. vesca 'Hawaii 4' and octoploid F. × ananassa 'Calypso'.

Results

Agrobacterium-mediated transformation of leaf and petiole explants was used for efficient stable integration of constructs expressing plant codon-optimised Cas9 and single guide sequences under control of the Arabidopsis U6-26 consensus promoter and t

SUBMITTER: Wilson FM 

PROVIDER: S-EPMC6495592 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature