Differences between karyotypically normal and abnormal human embryonic stem cells.

ABSTRACT:

Objectives

To compare different biological characteristics of human embryonic stem cells (HESCs) between those with normal and those with abnormal karyotype.

Materials and methods

Culture-adapted HESCs (chHES-3) with abnormal karyotype were compared with karyotypically normal cells, with regard to pluripotency and differentiation capacity, ultrastructure, growth characteristics, gene expression profiles and signalling pathways.

Results

We found a new abnormal karyotype of HESCs. We observed that chHES-3 cells with normal and abnormal karyotypes shared similarities in expression markers of pluripotency; however, karyotypically abnormal chHES-3 cells had a tendency for differentiation towards ectoderm lineages and were easily maintained in suboptimal culturing condition

SUBMITTER: Yang S 

PROVIDER: S-EPMC6495872 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature