Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions.

ABSTRACT:

Objectives

The aim of the present study was to improve efficiency of isolation and to optimize proliferative potential of human spermatogonial stem cells (SSCs) obtained from obstructive azoospermic (OA) and non-obstructive azoospermic (NOA) patients, and further, to characterize these cells for potential use in infertility treatment or study of reproductive biology.

Materials and methods

We have applied a cell-sorting method, using collagen and magnetic activated cell separation to overcome obstacles, developing a collection system, and simple long-term proliferation system, that yields large numbers of high-purity SSCs from obstructive OA and NOA patients.

Results

SSCs derived from OA and NOA patients proliferated and maintained their characteristics for more than 1

SUBMITTER: Lim JJ 

PROVIDER: S-EPMC6495878 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature