Identification of genes deregulated during serum-free medium adaptation of a Burkitt's lymphoma cell line.

ABSTRACT:

Objective

Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. Defined proteins and metal ions, such as insulin, growth factors, transferrin and sodium selenite, are sometimes also included and can in some cases substitute serum components. How adaptation to serum free media influences cells has not been studied in detail.

Materials and methods

We have adapted the Burkitt's lymphoma line Ramos to a serum-free medium that supports long-term survival and studied gene expression changes that occurred during the adaptation process.

Results and conclusions

The adaptation process was characterized by initial cell population growth arrest, and after that extensive ce

SUBMITTER: Zander L 

PROVIDER: S-EPMC6495949 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature