MiR-326/Sp1/KLF3: A novel regulatory axis in lung cancer progression.

ABSTRACT:

Objectives

To investigate the function and regulatory mechanism of Krüppel-like factor 3 (KLF3) in lung cancer.

Materials and methods

KLF3 expression was analysed by qRT-PCR and Western blot assays. The proliferation, migration, invasion, cycle and apoptosis were measured by CCK-8 and EdU, wound-healing and Transwell, and flow cytometry assays. The tumour growth was detected by nude mouse tumorigenesis assay. In addition, the interaction between KLF3 and Sp1 was accessed by luciferase reporter, EMSA and ChIP assay. JAK2, STAT3, PI3K and p-AKT levels were evaluated by Western blot and IHC assays.

Results

The results indicated that KLF3 expression was elevated in lung cancer tissues. Knockdown of KLF3 inhibited lung cancer cell proliferation, migration and invasion, and

SUBMITTER: Wang R 

PROVIDER: S-EPMC6495967 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature