MiR-709 up-regulated in hepatocellular carcinoma, promotes proliferation and invasion by targeting GPC5.

ABSTRACT:

Objectives

Hepatocellular carcinoma (HCC) is one of the most common cancers and is a significant leading cause of cancer-related deaths worldwide. Emerging evidence has shown that microRNAs (miRNAs) are associated with cancer development and progression. However, up to now little has been known concerning the role of miR-709 in HCC.

Materials and methods

Real-time RT-PCR was performed to detect expression of miR-709 in HCC cell lines and tissues. To further understand its role in HCC, we restored its expression in HepG2 cell line through transfection with miR-709 mimics or inhibitors. CCK-8 proliferation assay, migration assay and invasion assay were used to detect functional roles of miR-709. Luciferase assay and western blotting were performed to detect the target gene of

SUBMITTER: Liu T 

PROVIDER: S-EPMC6495981 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature