Expression pattern of Ngn3 in dairy goat testis and its function in promoting meiosis by upregulating Stra8.

ABSTRACT:

Objectives

Ngn3 is a typical transcription factor and marker of differentiating spermatogonial stem cells (SSCs) in mouse, belonging to the basic helix-loop-helix (bHLH) family. Its gene is specifically expressed in A type spermatogonia in mouse testis, thus plays a critical role in controlling differentiation of SSCs. However, roles of Ngn3 and its protein in dairy goat testis remain unknown.

Materials and methods

Testis development and expression patterns of Ngn3 were analysed by immunofluorescence and quantitative reverse transcription-polymerase chain reaction (QRT-PCR) in the dairy goat. Furthermore, effects of its overexpression on male germline stem cells (mGSCs) were evaluated by QRT-PCR, immunofluorescence, luciferase reporter assay and western blotting.

Results<

SUBMITTER: Tang F 

PROVIDER: S-EPMC6495982 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature